Elecsys insulin assay: free insulin determination and the absence of cross-reactivity with insulin lispro.
نویسندگان
چکیده
of 0.2 mgzkgzh are presented in Fig. 1, B and C, respectively. The concentration of SF recovered in the plasma sample was 0.80 mg/L. In conclusion, SPE before GC-MS represents a convenient method for quickly obtaining clean extracts with optimum recoveries and could be an accurate analytical tool for the monitoring of SF concentrations in plasma samples during pharmacokinetic studies. References 1. Van Daele PGH, De Bruyn MFL, Boey JM, Sanczuk S, Agten JTM, Janssen PAJ. Synthetic analgesics: N-(1-[2-arylethyl]-4-substituted 4-piperidinyl) N-arylalkanamides. Arzneimittelforschung 1976;26:1521–31. 2. Lemmens HJM. Opioids in intravenous anaesthesia. Anaesth Pharmacol Rev 1995;3:67–73. 3. Alibert F, Mangez J-F, Roux P, Bernstein D. Utilisation clinique des morphiniques en neuro-anesthésie. Agressologie 1991;32:435–7. 4. Michiels M, Hendriks R, Heykants J. Radioimmunoassay of the new opiate analgesics alfentanil and sufentanil. Preliminary pharmacokinetic profile in man. J Pharmacol 1983;35:86–93. 5. Woestenborghs R, Timmerman P, Cornelissen M-L, Van Rompaey F, Gepts E, Camu F, et al. Assay methods for sufentanil in plasma: radioimmunoassay versus gas chromatography-mass spectrometry. Anesthesiology 1994;80: 666–70. 6. Rovio S, Siren H, Riekkola M-L. Determination of sufentanil in human plasma by capillary electrophoresis and gas chromatography-mass spectrometry. J Liq Chromatogr Relat Technol 1997;20:1311–26. 7. Woestenborghs R, Michielsen L, Heykants J. Rapid and sensitive gas chromatographic method for the determination of alfentanil and sufentanil in biological samples. J Chromatogr 1981;224:122–7. 8. Szeitz A, Riggs KW, Harvey-Clark C. Sensitive and selective assay for fentanyl using gas chromatography with mass selective detection. J Chromatogr B 1996;675:33–42. 9. Rossi SS, Dyck JB, Yaksh TL. Simultaneous extraction of sufentanil and midazolam from human plasma. Clin Chim Acta 1996;244:103–10.
منابع مشابه
Performance evaluation and cross-reactivity from insulin analogs with the ARCHITECT insulin assay.
BACKGROUND Insulin measurement is used for the diagnosis of hypoglycemia and for insulin pharmacokinetic evaluations. We assessed the analytical and clinical performance of the ARCHITECT insulin assay, a chemiluminescent immunoassay recently introduced for the ARCHITECT i2000 fully automated immunoassay analyzer (Abbott Laboratories). We also tested whether major insulin analogs cross-reacted w...
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BACKGROUND Insulin assays are affected by varying degrees of interference from anti-insulin antibodies (IAs) and by cross-reactivity with recombinant insulin analogues. We evaluated the usefulness of the E170 insulin assay by assessing IA effects and cross-reactivity with 2 analogues. METHODS Sera were obtained from 59 type 2 diabetes patients receiving continuous subcutaneous insulin infusio...
متن کاملUse of a novel double-antibody technique to describe the pharmacokinetics of rapid-acting insulin analogs.
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Inhalational insulin was withdrawn from the market due to its potential to produce airway hyper-reactivity and bronchoconstriction. So the present study was designed to explore the acute effects of insulin on airway reactivity of guinea pigs and protective effects of salbutamol and beclomethasone against insulin induced airway hyper-responsiveness on isolated tracheal smooth muscle of guinea pi...
متن کاملComparative Study of Protective Effects of Salbutamol and Beclomethasone against Insulin Induced Airway Hyper-reactivity on Isolated Tracheal Smooth Muscle of Guinea Pig
Inhalational insulin was withdrawn from the market due to its potential to produce airway hyper-reactivity and bronchoconstriction. So the present study was designed to explore the acute effects of insulin on airway reactivity of guinea pigs and protective effects of salbutamol and beclomethasone against insulin induced airway hyper-responsiveness on isolated tracheal smooth muscle of guinea pi...
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عنوان ژورنال:
- Clinical chemistry
دوره 47 3 شماره
صفحات -
تاریخ انتشار 2001