Elecsys insulin assay: free insulin determination and the absence of cross-reactivity with insulin lispro.

نویسندگان

  • R Sapin
  • V Le Galudec
  • F Gasser
  • M Pinget
  • D Grucker
چکیده

of 0.2 mgzkgzh are presented in Fig. 1, B and C, respectively. The concentration of SF recovered in the plasma sample was 0.80 mg/L. In conclusion, SPE before GC-MS represents a convenient method for quickly obtaining clean extracts with optimum recoveries and could be an accurate analytical tool for the monitoring of SF concentrations in plasma samples during pharmacokinetic studies. References 1. Van Daele PGH, De Bruyn MFL, Boey JM, Sanczuk S, Agten JTM, Janssen PAJ. Synthetic analgesics: N-(1-[2-arylethyl]-4-substituted 4-piperidinyl) N-arylalkanamides. Arzneimittelforschung 1976;26:1521–31. 2. Lemmens HJM. Opioids in intravenous anaesthesia. Anaesth Pharmacol Rev 1995;3:67–73. 3. Alibert F, Mangez J-F, Roux P, Bernstein D. Utilisation clinique des morphiniques en neuro-anesthésie. Agressologie 1991;32:435–7. 4. Michiels M, Hendriks R, Heykants J. Radioimmunoassay of the new opiate analgesics alfentanil and sufentanil. Preliminary pharmacokinetic profile in man. J Pharmacol 1983;35:86–93. 5. Woestenborghs R, Timmerman P, Cornelissen M-L, Van Rompaey F, Gepts E, Camu F, et al. Assay methods for sufentanil in plasma: radioimmunoassay versus gas chromatography-mass spectrometry. Anesthesiology 1994;80: 666–70. 6. Rovio S, Siren H, Riekkola M-L. Determination of sufentanil in human plasma by capillary electrophoresis and gas chromatography-mass spectrometry. J Liq Chromatogr Relat Technol 1997;20:1311–26. 7. Woestenborghs R, Michielsen L, Heykants J. Rapid and sensitive gas chromatographic method for the determination of alfentanil and sufentanil in biological samples. J Chromatogr 1981;224:122–7. 8. Szeitz A, Riggs KW, Harvey-Clark C. Sensitive and selective assay for fentanyl using gas chromatography with mass selective detection. J Chromatogr B 1996;675:33–42. 9. Rossi SS, Dyck JB, Yaksh TL. Simultaneous extraction of sufentanil and midazolam from human plasma. Clin Chim Acta 1996;244:103–10.

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عنوان ژورنال:
  • Clinical chemistry

دوره 47 3  شماره 

صفحات  -

تاریخ انتشار 2001